PCR:	ATG wt
Name of Positive CTRL:	ATG wt
Annealing Temperature:	65.0 °C
Number of cycles:	40.0
Extension Time:	60 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	ex3-1	170	100 µM	20.0 µl for 1ml	gaatatgaaggcacacccctgaaatg
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
	short2	66	100 µM	20.0 µl for 1ml	gtactgcataatggtttaactcttgc
Expected Bands:	Band Size: 249.0	Description: 249mTUBB	(text displayed in the image)
	Band Size: 350.0	Description: 350wt	(text displayed in the image)
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